Here's a list of some folders you may definitely want to back up:
Your e-mail folders:
Microsoft Outlook usually resides in C:\Documents and Settings\Username\Local Settings\Application Data\Microsoft\Outlook
Microsoft Outlook Express will be in C:\Documetns and Settings\Username\Application Data\Identities\
Mozilla Thunderbird will generally be in C:\Documents and Settings\Username\Local Settings\Application Data\Thunderbird\
Your Windows address book (C:\Documents and Settings\Username\Application Data\Microsoft\Address Book)
Your fonts folder (C:\Windows\Fonts)
Your Desktop Folder (C:\Documents and Settings\Username\Desktop)
Username in these folder paths above always designates whatever name you use to log in to your computer. So if your name is Bob, the path for your Desktop folder will be C:\Documents and Settings\Bob\Desktop.
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